Lee Yx

4 Questions 2 Answers 0 Followers

Questions related from Lee Yx

Whether EGF induced signalings are continuously activated in cells cultured with serum-supplemented medium ?

Since the fetal bovine serum contains growth factors such as EGF，I am wondering whether the EGF induced signalings are continuously activated in cells cultured with serum- supplemented medium?...

15 November 2022 8,508 1 View

Does anyone have experience with extraction of membrane associated proteins after crosslinking?

The article described that cross-linkers are useful for studying structure and function of membrane proteins and cross-linking will locate various proteins on both sides of a membrane. But I do...

18 May 2022 4,040 0 View

Does HepG2 cells express E-cadherin or other cadherin family proteins?

I can not detect expression of E-cadherin in HepG2 cells by immunofluorescence and western blot in our lab. Also no signal is detected when pan-cadherin antibody is used in immunofluorescence. But...

08 December 2021 6,227 1 View

I performed a western transfer and see the appearance of diffuse bands on the membrane . What could have happened?

I performed a western transfer and see the appearance of diffuse bands on the middle of the membrane . The pattern was normal after electrophoresis.

27 September 2021 2,837 4 View