Laura Kahn

5 Questions 4 Answers 0 Followers

Questions related from Laura Kahn

Does DNA denaturing with 2N HCl affect DAPI staining in whole mount assay?

I'm trying a whole mount assay with BrdU. I've read that people have issues with DAPI staining after treating their slides with HCl for antigen retrieval. This is a whole mount assay, as I've...

08 March 2017 8,429 2 View

Is there a way to sterilize poly-L-lysine?

I have some poly L lysine which isn't sterile (we use it to coat slides por microscopy) and I would like to use some for cell culture, so my cells can attach to the culture bottle. Is there a way...

14 September 2016 2,184 3 View

What's the correct recipe for Fekete's solution?

The current protocols of immunology says this: Fekete's solution For 1 liter: 580 ml 95% ethanol 200 ml H2O 80 ml 37% formaldehyde solution 40 ml glacial acetic acid...

02 April 2014 6,049 6 View

What is the difference between collagenase type I and type IV?

I'm looking for a protocol to isolate T cells from a melanoma tumour. I've found several but in some they use collagenase type I and in others type IV. What is the difference? Should type I be...

06 March 2014 8,726 7 View

How can I do a tumor lysate?

I'm looking for a protocol for tumor lysate. An easy, low cost one. A protocol for tissues would work?

12 November 2013 6,782 5 View