Ladan Kalani

6 Questions 7 Answers 0 Followers

Questions related from Ladan Kalani

What do the ghost peaks in this RP-HPLC correspond to?

Hello, I have performed NaCl extraction of a nuclear protein and run it on RP-HPLC with a mobile phase of acetonitrile in 0.1% TFA in water. I had repeatedly observed two tall peaks around minute...

14 April 2022 1,858 0 View

What causes the horizontal smear on neighboring wells of this western?

Hello, I am encountering a strange horizontal smear of neighboring wells of my recent westerns. Now protein quantification is problematic. I am unsure whether this is due to some horizontal...

06 October 2021 1,114 4 View

What could bind to ubiquitin monoclonal antibody in nuclei from brain tissues and HEK cells that has MW of ~ 37kDa?

Hello, I have purchased ubiquitin monoclonal antibody Ubi 1: Ubiquitin Antibody (13-1600) Thermofisher; I intended to check the quality of this monoclonal antibody and I run some brain nuclei at...

24 April 2021 2,467 0 View

Has anyone tried growing C2C12 cells in 1X DMEM without L-glutamine?

Hello, I plan to transfect C2C12 cells and I am happy if their growth rate would slow down if they are not provided with glutamine (as they grow so fast)! Has anyone tried growing C2C12 cells in...

06 March 2021 664 3 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

04 February 2021 9,610 5 View

When sequencing a plasmid to confirm transformation has been successful, would I have to sequence the full plasmid?

I have transformed some DH5alpha cells with point mutated proteins placed in pcDNA plasmid and extracted the plasmid; now I have to send the plasmid for sequencing for confirming the presence of...

20 January 2021 6,070 3 View