L. Miguel Martins

6 Questions 9 Answers 0 Followers

Questions related from L. Miguel Martins

What are the statistics involved in dividing an expression ratio by another?

I am trying to reproduce a methodology described to identify changes in gene translation ratios using microarray experiments described in PubMed ID19804760, page 158. To calculate translational...

09 September 2015 1,518 1 View

Does mitofusin promote or inhibit contacts between mitochondria and the endoplasmic reticulum?

A recent report by Filadi and colleagues reports that removal of mitofusin increases mito-ER contacts. This is interesting but puzzling. Schneeberger and colleagues (PubMed ID 24074867) also...

05 May 2015 3,384 6 View

Is it possible to separate ribosomes that selectively translate nuclear-encoded mitochondrial proteins from those translating cytosolic proteins?

Import of nuclear encoded-mitochondrial proteins into these organelles is coupled to translation. Can you suggest a biochemical method that would allow us to separate ribosomes actively...

06 June 2014 3,188 2 View

Any thoughts on the "Simple Western" system for protein simple as an automated and quantitative western assay?

I have tried other quantitative systems in the past but always felt the old-fashioned wet transfer and ECL with film detection are far superior to any more modern alternatives. I was sent some...

04 April 2014 2,913 2 View

Can one perform whole exome sequencing from whole genome amplified material from FFPE? Any thoughts on this?

Exome sequencing from FFPE (formalin-fixed paraffin embedded tissues)

02 February 2014 6,632 0 View

Do any of you know of primer cocktails to perform deep sequencing of Drosophila mitochondrial genome using semiconductor technology (IonTorrent)?

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Sequencing/Semiconductor-Sequencing/Applications-Semiconductor-Sequencing/Mitochondrial-Sequencing.html

01 January 2013 4,487 1 View