Kyla Bourque

2 Questions 2 Answers 0 Followers

Questions related from Kyla Bourque

Can i use primers designed for genomic DNA to amplify cDNA?

I need to design primers for cDNA however, the genes that i want to amplify, I am not sure if they are expressed ( i am doing a time course and they may not be expressed at the beginning but...

16 March 2018 6,504 6 View

When using the Cre-lox system for site specific integration, what are the chances that my GOI inserts at a random unwanted site?

Does anyone know of a good paper that discusses possible off-target effects of the Cre-lox system? And how to screen for this in iPS cells? Would i need to do clonal selection after the...

24 January 2018 9,779 0 View