Kyla Bourque

3 Questions 3 Answers 0 Followers

Questions related from Kyla Bourque

Can i use primers designed for genomic DNA to amplify cDNA?

I need to design primers for cDNA however, the genes that i want to amplify, I am not sure if they are expressed ( i am doing a time course and they may not be expressed at the beginning but...

16 March 2018 6,568 6 View

When using the Cre-lox system for site specific integration, what are the chances that my GOI inserts at a random unwanted site?

Does anyone know of a good paper that discusses possible off-target effects of the Cre-lox system? And how to screen for this in iPS cells? Would i need to do clonal selection after the...

24 January 2018 9,822 0 View

Which protocol would you suggest for iPSC differentiation into cardiomyocytes? Using RPMI-B27 or using the chemically defined 3 component media CDM3?

Differentiation based on Wnt modulation, however there are different media that are used in the process? Which compositions are most efficient? Some use RPMI-B27 minus insulin supplement, other...

29 November 2017 2,762 3 View