Differentiation based on Wnt modulation, however there are different media that are used in the process? Which compositions are most efficient? Some use RPMI-B27 minus insulin supplement, other have chemically defined media using recombinant human albumin and ascorbic acid 2-phosphate? What would you recommend?
Well, What I do is to differentiate usign RPMI1640-B27 and then purifying them with the CDM3. This way you will get a reasonably pure cardiomyocyte population.
Okay! Which method is least expensive? You dont do lactate method for selection?
I do lactate them for selection.
The medium and stuff are not so expensive. I would imagine it is affordable by any biology lab.
We do starvation at day 9 until day 11. Differentiated cardiomyocytes usually start beating after 6-8 days.
- Badges
- Science topic
- Similar topics
- Computer Science and Engineering
- Artificial Intelligence