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Questions related from Konstantin Byrgazov
Hi! I am interested in comparing expression of a particular single gene between metastatic osteosarcoma and non-metastatic disease. There are plenty of array data in GEO. I have downloaded the...
08 January 2019 8,554 0 View
Hi everyone! Recently I was doing a lot screening of drug pairs in 5 osteosarcoma cell lines using this amazing website https://synergyfinder.fimm.fi. I am puzzled why a lot of combinations have...
09 May 2018 9,213 3 View
I am going to perform a CRISPR/Cas-mediated knock-in. Unfortunately, a donor vector which I have has a C>T SNP right at the middle of one of the homology arms. The SNP is at position 400...
05 January 2017 5,457 1 View
I have the protein structure and would like to design the small molecule compounds to interfere with the protein function. I know which pocket to target. Last time I was in medicinal chemistry was...
19 May 2016 7,904 6 View
I seek for your advice on the strategy on 5' RACE if I have a sample with cDNA of the patient. As I understood from the literature search, I should start with the 5' priming of the cDNA. In this...
19 February 2014 1,703 5 View
Dear fellows, I am perplexed with some DNA damage response in my osteosrrcoma cell lines. I am treating the cells with the same concentrations of three cytotoxic agents: a novel melphalan...
01 January 1970 629 2 View
