Kiran Bobde

12 Questions 19 Answers 0 Followers

Questions related from Kiran Bobde

How to increase algal cell wall permeability for drug?

Independent on type of drug. Is any other chemical like DMSO, ethanol, Nanopartical or Can I use CaCl2. Can please suggest me any other type of chemical or solvent which help for increasing cell...

24 May 2017 8,519 5 View

Why GC data shows two different peaks and retention times for same compound?

During GCMS analysis, it is showing my sample contains Squalene. But whenever I am running single standard (Squalene ≥ 98% purity) as well as internal standard compound sometimes show peak and...

16 January 2017 954 9 View

How to make a photobioreactor at lab scale (Batch and semi-batch reactor)?

- Light, DO, Inoculum size which parameter should I need to be a focus? - Can please give me literature on basic designing of a reactor?

02 August 2016 6,762 4 View

What kind of info we will get by the measurement of conductivity during microalgal growth in modified BG-11?

During the growth kinetics study conductivity negativity is increase with respect to days of incubation. As a biological i am not reaching to any conclusion? How this info help to my microalgal...

25 September 2015 3,627 2 View

Why does botryococcus braunii kept at 4°C settle on the bottom of the culture flask?

Is there any changes is inclusion bodies/gas vacuoles? Is this system useful for Sole Hydrocarbon production? or Is some cheaper way of harvesting of B. braunii?

10 August 2015 1,463 1 View

What is best method of drying total lipid content during the gravimetric analysis?

Right now according to different literature mostly gaseous nitrogen used for drying extract and some of them they were using the hot air oven (50 degree centigrade) for drying lipid extract...

28 July 2015 9,754 4 View

Does anyone know how I can increase permeability of a eukaryotic cell?

I am using different chemical like Ethanol, H2O2, toluene, ether, phenylethyl alcohol DMSO, benzene, methanol, chloroform.

05 February 2015 5,085 6 View

What is the flash point of chloroform?

This organic solvent is useful for Lipid extraction from microalgal biomass.

24 January 2015 1,127 3 View

What can be used as a preservative for microalgae in a freshwater sample?

Other than 2-5% as formalin and glutaraldehyde.

10 October 2014 833 6 View

Is there any method for the reduction of the doubling time other than Genetic Engineering or Media optimization?

I am working with eukaryotic systems of Microalgae.

05 October 2014 8,510 7 View

How can I fix microalgal cells during Nile red staining?

I am working with an indigenous strain of microalgae (Scenedesmus sp. , Botryococcus braunii, Chlorella sp.). During fluorescence microscopy analysis the cells were floating under the microscope....

09 September 2014 4,851 4 View

Why does microalgae induce lipids under stress condition?

In general, microalgae lipid is used as reserve food material in (2nd source of energy ) and that energy will be used for survive in unfavorable condition.

08 March 2014 8,227 9 View