Kim Chen

3 Questions 3 Answers 0 Followers

Questions related from Kim Chen

Why PEGDA (Poly(ethylene glycol) diacrylate) spread outside hydrophobic wax barrier?

I'm working on photopolymerization on Whatman No.1 chromatography paper. I printed a circle using a toner laser printer and heated it inside the oven at 180 degrees Celsius for 1 hour to create...

19 April 2022 6,924 0 View

Why polymerization amplification solution will spread outside hydrophobic wax barriers?

I created a hydrophobic wax barrier by printing a circle using a laser printer and putting it under the oven at 165 degrees Celcius for 15 minutes. I tried the hydrophobic wax barrier with a few...

15 April 2022 231 1 View

Should I perform qPCR assay optimization first or assay performance?

Hi all, I m new to qPCR. Currently, I would like to perform assay optimization (primers & probe concentration optimization) and assay performance (serial dilution of cDNA). However, I am...

09 September 2019 9,353 6 View