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Questions related from Samima Khatun
Hi everyone, [D]t={(F0-F/F)*(1/Kb)}^(1/n)+n*[P]t*(F0-F/F) where [D]t and [P]t gives total drug conc and total protein conc. we want to determine Kb and n. we have a series of data for y=(F0-F/F),...
08 September 2018 921 3 View
We have used FRET method to see the donor(protein)- acceptor(drug) distance which we get for our system as r=1.6 nm. Docking we perform to find out binding site of that particular drug in protein...
09 July 2018 2,869 2 View
Hi everyone We generally use CD and FTIR techniques to see the secondary structure change of protein in presence of drug. Docking we perform to find out binding site of that particular drug in...
09 July 2018 7,365 2 View
For a drug (suppose X) the thermodynamic parameters for BSA-X system from fluorescence method was found to be hydrogen bonding and van der waals forces while for the Lysozyme-X system the forces...
09 July 2018 6,995 7 View
Dear all I have performed ITC for protein and ligand solution.generally we substract heat of dilution of ligand from overall heat for ligand titration into HSA .is it necessary to substract the...
31 May 2017 4,375 4 View
Dear all is it compulsory that the thermodynamic parameters we got from fluorescence technique at different temperature must be same as we got from ITC technique? for example we get dH and dS...
31 May 2017 9,462 3 View
Fluorescence result of a HSA-drug system proved that hydrophobic result is the main force .In docking both hydrogen and hydrophobic force are there.but ITC result for the same system give...
31 January 2017 258 5 View
