Samima Khatun

13 Questions 34 Answers 0 Followers

Questions related from Samima Khatun

How to fit a non-linear equation?

Hi everyone, [D]t={(F0-F/F)*(1/Kb)}^(1/n)+n*[P]t*(F0-F/F) where [D]t and [P]t gives total drug conc and total protein conc. we want to determine Kb and n. we have a series of data for y=(F0-F/F),...

08 September 2018 1,018 3 View

Can we correlate our FRET result (r=1.6 nm) with molecular docking ?

We have used FRET method to see the donor(protein)- acceptor(drug) distance which we get for our system as r=1.6 nm. Docking we perform to find out binding site of that particular drug in protein...

09 July 2018 2,922 2 View

Is CD and FTIR based secondary structure change confirmed by docking?

Hi everyone We generally use CD and FTIR techniques to see the secondary structure change of protein in presence of drug. Docking we perform to find out binding site of that particular drug in...

09 July 2018 7,452 2 View

What are the possible reason for discrimination between fluorescence and docking result?

For a drug (suppose X) the thermodynamic parameters for BSA-X system from fluorescence method was found to be hydrogen bonding and van der waals forces while for the Lysozyme-X system the forces...

09 July 2018 7,051 7 View

How to perform competitive binding study by ITC ?

Hellow everyone, how to perform competitive binding study by ITC ? means the steps follow in this study? i want to know the binding site of a drug on BSA protein by using known site marker like...

07 November 2017 7,044 12 View

How to calculate error and standard deviation (SD) for experimental data?

Dear all for fluorescence data , for % of alpha helix or for any other tech how to calculate error in data ,SD for accuracy.is there any freely available software to calculta it ?plz help me thanks

03 June 2017 1,562 6 View

Substraction of heat of dilution from ITC result ?

Dear all I have performed ITC for protein and ligand solution.generally we substract heat of dilution of ligand from overall heat for ligand titration into HSA .is it necessary to substract the...

31 May 2017 4,433 4 View

Difference in thermodynamic parameters between ITC and Fluorescence techniques ?

Dear all is it compulsory that the thermodynamic parameters we got from fluorescence technique at different temperature must be same as we got from ITC technique? for example we get dH and dS...

31 May 2017 9,517 3 View

Can drug in a organic solvent give ITC ?

i want to do ITC experiment of protein (which is in phosphate buffer,water soluble) with a drug (which is soluble in organic solvent ).is this ok? or organic solvent can create a problm ?

02 February 2017 6,464 4 View

How to explain ITC result ?

Fluorescence result of a HSA-drug system proved that hydrophobic result is the main force .In docking both hydrogen and hydrophobic force are there.but ITC result for the same system give...

31 January 2017 315 5 View

Can ITC give different result for HSA and BSA with same drug ?

i did ITC experiment for HSA and BSA with a same drug .and keeping conc same for both case.Whereas HSA give exothermic BSA give endothermic.is it possible ?

31 January 2017 3,658 4 View

How one can increase the solubility in water ?

i am working with an insoluble drug....i want to increase its solubility in water by a simple and easy technique like adding 20% of methanol to it or by shaking/heating process.or something like...

03 March 2016 7,438 12 View

Why my drug gives its peak near 250 nm instead of 280 nm of native HSA?

I have done my uv-visible experiment where native HSA give its peak at 280 nm..bt after adding drug the peak maximum is at 250 nm instead of 280 nm..what is the reason behind this ?

02 March 2016 2,607 5 View