Khalid Eldahan

4 Questions 15 Answers 0 Followers

Questions related from Khalid Eldahan

How quickly are lentiviral vectors transported in the central nervous system?

I'm trying to estimate the amount of transport time needed to map out specific circuits in the spinal cord using a rabies-glycoprotein pseudotyped lentiviral vector. Does anyone know the kinetics...

19 January 2017 2,825 1 View

Has anyone cultured upper or lower motor neurons from rat spinal cord?

I am interested in testing pharmacological agents on sensory and motor neurons. My experience with cell culture is limited to aggressive tumor cells which culture quite easily. I have two...

16 July 2015 1,399 1 View

What are the important considerations in detecting large M.W. proteins using SDS-PAGE western blot?

I am intersted in detecting the p-mTOR protein, which is around 290 kDa. Can anyone give me some tips on how to efficiently blot for proteins in this size-range? At the moment, I am using 4-20%...

14 July 2015 1,525 6 View

Has anyone used immunofluorescence to detect phosphoproteins on cryosectioned tissues?

I have used western blot to detect phosphoproteins and am aware that it is important to treat samples with phosphatase inhibitors (Sodium fluoride, Orthovanadate etc.) to stabilize...

02 July 2015 8,167 3 View