Khalid Eldahan

6 Questions 19 Answers 0 Followers

Questions related from Khalid Eldahan

How quickly are lentiviral vectors transported in the central nervous system?

I'm trying to estimate the amount of transport time needed to map out specific circuits in the spinal cord using a rabies-glycoprotein pseudotyped lentiviral vector. Does anyone know the kinetics...

19 January 2017 2,891 1 View

Does anyone have experience implanting radio-telemetry devices into rats to record hemodynamics directly from the descending abdominal aorta?

If so: Do you observe animal mortality in the hours/days following the implantation surgery? What seems to be the cause of death in those cases? (i.e. blood loss, ischemic damage, embolism) From...

03 December 2015 7,636 6 View

Has anyone cultured upper or lower motor neurons from rat spinal cord?

I am interested in testing pharmacological agents on sensory and motor neurons. My experience with cell culture is limited to aggressive tumor cells which culture quite easily. I have two...

16 July 2015 1,444 1 View

What are the important considerations in detecting large M.W. proteins using SDS-PAGE western blot?

I am intersted in detecting the p-mTOR protein, which is around 290 kDa. Can anyone give me some tips on how to efficiently blot for proteins in this size-range? At the moment, I am using 4-20%...

14 July 2015 1,566 6 View

Has anyone used immunofluorescence to detect phosphoproteins on cryosectioned tissues?

I have used western blot to detect phosphoproteins and am aware that it is important to treat samples with phosphatase inhibitors (Sodium fluoride, Orthovanadate etc.) to stabilize...

02 July 2015 8,212 3 View

Has anyone successfully stained rat spinal cord cross-sections with an antibody against p-NR1?

I have encountered issues performing fluorescent IHC on rat spinal cord cross-section to detect p-NR1 (SER 896). The antibody used in "ABN88" from millipore. While I do see positive signal...

11 June 2015 2,211 2 View