Kelly Velasco

4 Questions 4 Answers 0 Followers

Questions related from Kelly Velasco

How to prepare a alpha-ketoglutarate solution and store it?

Hi, I will do an enzymatic assay using a-ketoglutarate as substrate and I am wondering about the proper way to prepare and store this compound. As long as I could find out, it would be OK to...

09 September 2018 902 3 View

Which solvent should I use to dissolve Acetoacetyl CoA and how should I store the solution?

Hi! I am performing and enzymatic assay, where Acetoacetyl-CoA is the substrate. I have gotten the Acetoacetyl-CoA powder that SIGMA sells (5 mg). I think it would be easier to handle if I just...

03 March 2015 3,733 5 View

Which is the right way of reporting the data from a continuous coupled enzymatic assay?

Hi! I am measuring the activity of my enzyme of interest through the decrease on the absorbance at 340 nm (to detect the disappearance of NADH). I am measuring every minute for 8 minutes, and I am...

03 March 2015 2,899 5 View

Why do I get a smear when using a TNT cell free expression system to express my plasmid?

Hi! I am trying to express my protein of interest in a TNT cell free expression system (Promega, L1170). My protein is tagged With V5 and His tags. The problem is when I run the samples in a...

12 December 2014 9,490 4 View