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Questions related from Keh Chien Lee
Dear Experts, I am working to identify/confirm RNA that binds to my protein of interest through RNA-ChIP assay. However, i have no experience at all in ChIP assay and its not establish in my lab....
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Dear Experts, I am working on RT-PCR analysis on arabidopsis genes. I notice that recently when i isolate RNA and convert it to cDNA, some sample give no/low amplification of housekeeping...
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Dear Researcher, First of all, thanks for reading my post. As i am working with a Arabidopsis knockout mutant that showed several phenotypes; one of it showed higher survival rate after heat...
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Hi guys. thanks for reading. I want to make transgenic line with my gene of interest using its own promoter with and without GUS reporter gene. My first question is if iclone in my gene of...
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Hi guys. i am checking heat resistancy/susceptibility of my arabidopsis mutant. First i grow seeds on MS for 1 week and do heat treatment at 45 degree for 70 mins and allow it to recover for 3...
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