Keh Chien Lee

5 Questions 5 Answers 0 Followers

Questions related from Keh Chien Lee

Inquiries of RNA-Chromatin immunoprecipitation?

Dear Experts, I am working to identify/confirm RNA that binds to my protein of interest through RNA-ChIP assay. However, i have no experience at all in ChIP assay and its not establish in my lab....

06 July 2016 7,645 3 View

Why would myRNA concentration be good, but low/no amplification from housekeeping genes?

Dear Experts, I am working on RT-PCR analysis on arabidopsis genes. I notice that recently when i isolate RNA and convert it to cDNA, some sample give no/low amplification of housekeeping...

24 September 2015 4,599 2 View

Any suggestions on my enquiry of Arabidopsis heat stress treatment?

Dear Researcher, First of all, thanks for reading my post. As i am working with a Arabidopsis knockout mutant that showed several phenotypes; one of it showed higher survival rate after heat...

20 March 2015 6,090 5 View

Can anybody help me designing my GUS reporter constructs?

Hi guys. thanks for reading. I want to make transgenic line with my gene of interest using its own promoter with and without GUS reporter gene. My first question is if iclone in my gene of...

06 February 2015 5,305 2 View

Can someone advise a definite clarification on survival rate of arabidopsis heat treatment?

Hi guys. i am checking heat resistancy/susceptibility of my arabidopsis mutant. First i grow seeds on MS for 1 week and do heat treatment at 45 degree for 70 mins and allow it to recover for 3...

03 February 2015 3,033 2 View