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Questions related from Keagan Dunville
Has anyone used plasmids from "A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins." A gene-fusion strategy for stoichiometric and co-localized...
05 May 2018 2,529 0 View
Hi all, Does anyone know the following: a company or lab which can generate MAPT knockout of murine embryonic stem cells? an already established MAPT KO mESC line*? or a preferred/robust Cas9...
05 May 2018 2,278 2 View
Hi all, This question is a bit naive. I was under the impression that AT8 recognizes pSer202 & pThr205 but does not necessarily indicate tau aggregation. However, a collaborator informed me...
01 January 2018 6,964 3 View
Hi all, I'm currently attempting to differentiate feeder-free hiPSCs to cortical neurons, however, I'm having problems with simply expanding the cell line to an appropriate amount. The major...
11 November 2017 4,348 0 View
Currently attempting to expand hIPSCs after thawing. Is there a basement coating for Geltrex that sustains feeder-free hIPSCs better? My current protocol (briefly) looks like this: Well...
03 March 2017 8,937 2 View