Kathy Y Wei

3 Questions 3 Answers 0 Followers

Questions related from Kathy Y Wei

Human rRNA promoter sequence?

I am looking for the human rRNA promoter sequence, but am having trouble finding a specific sequence. What I did find was in Jones et al 1988 PNAS Analysis of clustered point mutations in the...

12 December 2013 2,980 1 View

Does VP64 enhance expression from CMV promoter?

I am trying to enhance expression of my cassette and recently learned about VP16 and VP64 for activating gene expression. However, these seem to be used with a minimal CMV promoter rather than a...

07 July 2013 4,898 0 View

Change in phenotype, but no change in mRNA when using siRNA

I did a dilution curve with an siRNA to look at phenotype (cell cycle distribution by flow cytometry), as well as mRNA levels (by qRT-PCR) in U2-OS cells, expecting to see decreasing mRNA levels...

04 April 2013 922 5 View