Katherine Balasingham

4 Questions 15 Answers 0 Followers

Questions related from Katherine Balasingham

How do you obtain all consensus exon sequences from WES?

After bwa assembly to the reference genome I end up with sorted and indexed BAM files, but I am interested in understanding how to properly obtain all exon consensus sequences (to produce...

10 October 2019 5,737 2 View

No amplification for 400bp, but can easily get amplification for 700+bp?

Bit of a puzzling scenario here for some samples... DNA samples extracted from water using BioBasic soil kits, will amplify 16S rRNA(~400bp), and ~700bp region of COI gene very well. However, the...

01 January 2016 4,934 7 View

Why won't my PCR Primers amplify DNA extracted from a kit but will amplify all other samples?

I have different samples of Atlantic salmon. I have PCR primers (amplify region of ~280bp) which worked on eDNA samples of ATL (I gathered water from a tank filled with ATL and extracted via PCI),...

07 July 2015 4,890 8 View

What is the best method to clean ~30ul of DNA?

After extracting DNA from very dirty water samples doing a double PCI extraction (with CTAB) - pellets were brown. Rehydrated samples in 30ul of TE buffer but they have a light brown tinge to it -...

03 March 2015 1,027 7 View