Kassondra Hickey

6 Questions 6 Answers 0 Followers

Questions related from Kassondra Hickey

What could be the cause of the turbidity in my striated muscle explant cultures?

I am performing explants on marine invertebrates and out of all of the organs, the striated muscle specifically has yellowing media with turbidity. The bottom of the tissue culture plate seems to...

02 May 2022 1,953 1 View

Why does my microfluidic flow focusing device switch between large droplets and many small droplets?

I am using an MFFD to create hyaluronic acid microgels. While I have been able to get stable flow over the course of 4 hours, I seem to get alternating flow patterns which generate either many...

12 June 2019 6,341 5 View

For microfluidic flow focusing devices, how do you prevent backflow of continuous phase into the dispersed phase during stabilization?

Hi all, working on making microgels with a microfluidic flow focusing device. How long do you let your device stabilize before introducing the dispersed phase (I know this is device dependent)?...

17 November 2018 5,867 0 View

Why are my primary mixed glia not proliferating?

I have isolated primary mixed glia from P0-P2 mouse cortices and use trypsin for dissociation. I am trying to expand my cultures for qPCR experiments. So far I have not had luck with my cell...

09 March 2018 3,878 6 View

How long can primary cells be cultured and maintain similar qPCR Ct values?

I am culturing bEnd.3 cells (mouse brain endothelial cells). I have noticed my Ct values for the same cDNA input amount increase. For example, GAPDH went from Ct 13 to 20 when using cells from...

01 November 2017 6,270 3 View

Are there any beginner tips on stabilizing a mouse with jaw cuffs for stereotaxic surgery?

I am new to stereotaxic surgery and looking for any tips or ideas to help me get the mouse head stable and level using jaw holder cuffs. Are there any ways to identify proper placement quickly?

03 October 2017 4,361 1 View