Kaspar Valgepea

4 Questions 39 Answers 0 Followers

Questions related from Kaspar Valgepea

Shipping of a purified protein as precipitate or as freeze-dried at ambient temperature?

I have to ship a batch of purified proteins at ambient temperature to a collaborator. The functional state, activity, and folding of the proteins after shipping are not important as they will be...

03 March 2018 5,418 5 View

Could anyone point me to data of wild-type E. coli populations showing how length and width of single cells changes with specific growth rates?

I am looking for a data set which has analyzed this question with wild-type E. coli populations. I am aware of "old works" where few wild-type cells were analyzed using microscopy and newer...

01 January 2014 8,202 28 View

Could someone point me to a data set containing acetate secretion rates in E. coli wild-type cultures growing faster than specific growth rate 0.8?

I have data for E. coli K-12 BW25113 up to specific growth rate 0.8 h-1, but I would need acetate secretion rates or acetate yields on glucose or biomass at faster growth for modeling purposes....

12 December 2013 972 2 View

What is the most accurate and precise method to measure total protein concentration in (dry) biomass of bacteria?

Over the years in our lab, we have used different methods to determine the total protein concentration in dry biomass of different bacteria. Mostly the widely-used Lowry and Biuret methods with...

11 November 2013 5,433 12 View