Kamal Mandal

4 Questions 1 Answers 0 Followers

Questions related from Kamal Mandal

How to clean-up peptides labelled using click chemistry?

I am labelling my peptides using click chemistry but end up having brown residual pellet upon desalting (peptide clean-up). This contaminant leads to artifactually high nanodrop reading and...

18 May 2021 7,703 2 View

Are human primary macrophage cells fully adherent or partially?

I am trying to culture human primary macrophage using AIM-V media with GM-CSF. Even after day 10, I still see suspension cells. Is it something normal? I do see lot of adherent cells too but they...

24 June 2020 7,359 0 View

Epitope tag in internal region and not in C or N terminal end?

Does epitope tags such as FLAG, HA, c-myc etc work when positioned in internal region of protein and not in N or C terminal? I guess we need some spacer sequence flanking the tag to avoid stearic...

02 January 2020 4,795 0 View

Human primary macrophage cells are fully adherent or partially

I am trying to culture human primary macrophage using AIM-V media with GM-CSF. Even after day 10, I still see suspension cells. Is it something normal? I do see lot of adherent cells too but they...

01 January 1970 2,304 0 View