Kai-Lin Mao

8 Questions 7 Answers 0 Followers

Questions related from Kai-Lin Mao

Is guide cannula implantation a credibel method to deliver drugs to the mouse brain?

We conduct the guide cannula implantation surgery on mice lateral ventricle of the brain to deliver our drugs directly to the brain region. However, the mortality of this surgery is pretty high,...

12 November 2021 9,789 3 View

Can I use only one alcohol bottle to replace Intermittent Access to 20% Ethanol 2-Bottle Free-Choice (IA2BC) Drinking Procedure on mice?

I'm construction mouse alcohol addiction model to study alcohol addiction and withdrawal symptoms, many papers said they used Intermittent Access to 20% Ethanol 2-Bottle Free-Choice (IA2BC)...

12 November 2021 5,685 2 View

Single injection or repeated injection in the behaviour test?

I'm conducting behavior tests on mice, including the Open field test, elevated plus maze, and forced swimming test. There is a 3-day time interval between these tests, so in order to test the...

26 October 2021 652 3 View

Can I use the same protein sample of ELISA to perform Western-blotting?

I plan to detect the neurotransmitters and proteins in the mouse brain using ELISA and western blot, these two methods all test proteins in the sample, therefore I want to know if I can use the...

21 October 2021 4,005 3 View

How many times should I inject the drug before I take samples for RNA-Sequencing?

I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...

09 December 2020 5,006 2 View

How do I transform the "Transcripts" into the "Gene symbol"?

I downloaded the expression matrix data "GSE143416_Brain_data_TPM.txt.gz" of GSE143416 from Gene expression omnibus ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). However, the first column of...

01 December 2020 1,597 3 View

What does this melting curve tell?

I'm doing QRT-PCR, the melting curve looked like this, what can I know from this curve?

23 November 2020 7,027 5 View

QPCR: the amplification curve exists but no CT value, why?

I used the qTOWER 3 qpcr machine of JENA company to carry out my qPCR assay, after 45 cycles, the results showed the amplification curve and the gene did has been amplificated, however, no CT...

13 October 2020 1,647 4 View