Junwhee Yang

4 Questions 1 Answers 0 Followers

Questions related from Junwhee Yang

I get band smearing in sds-page when crosslink proteins. why?

Hello, I use homobifunctional crosslinkers to form protein gel. I am currently using 4-nitrophenyl carbonate leaving groups for protein crosslinking. I dissolve cross-linker in DMSO and slow add...

17 March 2020 9,509 3 View

Would it be possible to measure protein nano hydrogel with 90 plus brookhaven particle size analyser DLS?

Hello, I recently formulated bovine serum albumin nanogel with primary amine reactive protein crosslinkers. As I bmentioned in the title, I am using 90 plus brookhaven particle size analyser to...

12 December 2019 3,217 2 View

Are nanoparticles of protein aggregation formulated under organic solvent irreversibly denatured?

I formulated pure bovine serum albumin nanoparticle through desolvation method in ethanol. It seems like it turns clear after I add little bit of water after protein aggregations are dispersed in...

30 November 2019 1,851 0 View

What should I do to seperate cells from cell aggregations when culturing NIH3T3 cells?

Hello, I recently started a cell culture, but I have problem with the cell aggregation problem when detaching cell from a 100mm cell culture dish to another dish. After treating trypsin, I...

11 November 2019 6,584 4 View