7 Questions 6 Answers 0 Followers
Questions related from Jun Xing
Before doing western we always denature proteins in 95 degree for 5 mins. My question is for example: cyclin E always forms complex together will cdk2 in its functional form. So after 95 degree 5...
10 October 2017 4,340 2 View
Dear ALL; I am currently working with a natural compound which are effective to kill cancer cell in vitro. But the mechanism is unknown. I am eagerly to find out why and how the compound works....
06 June 2017 9,770 0 View
Dear All, Currently I am treating HCC tumor cell line in vitro using my special compounds. I am doing kinetic cell cycle experiment and I found that my drug gradual induce G2M block after 8-16hr...
06 June 2017 5,031 3 View
I am working on some new anti-cancer drug. In my in vitro experiment, After certain time point treatment of my drug I see obvious decrease in the level of protein AKT,pAKT and p-c-raf by western...
11 November 2016 2,027 4 View
Dear All, In my western blotting experiment I found a decrease level of both Akt and pAkt expression. So I want to check the mRNA level of them. I am wondering which primer should I buy for the...
10 October 2016 2,615 5 View
I want to staining the live cell colonies is soft agar assay. My lab we have INT(Iodonitrotetrazolium chloride) it's in solid state, the catalog is I8377 sigma. I want to know how to prepare it...
06 June 2016 6,647 4 View
I want to use western blot to check the protein level of p27, I use the protein ladder from Thermo Fisher. and the p27 antibody from cell signalling. I cut the membrane between 55kDa to 35kDa...
01 January 1970 4,881 10 View
