Julius Ryan Pronto

8 Questions 25 Answers 0 Followers

Questions related from Julius Ryan Pronto

Beta-tubulin staining shows striated staining pattern in cardiac sections?

I stained human atrial sections (1 µm) with CST #2146 ß-tubulin (1:75) but I sometimes get a striated staining pattern. Antigen retrieval was at pH 9. Blocking/permeabilisation with 0.1% Triton X...

26 March 2025 4,078 0 View

What is the appropriate duration of high-fat diet prior to STZ injection in making type 2 diabetic ApoE knockout mice?

Is 2 weeks of high fat diet too short before STZ injection (low dose, 5 days)? My mice are weaned at 4 weeks and acclimatized to a normal diet 1 week before subjecting them to a high fat diet....

05 March 2018 6,441 7 View

What is the most reliable housekeeping gene for western blot analyses using sarcoplasmic reticulum vesicles?

My target proteins are RyR2 and SERCA2 and their respective regulators.

07 March 2016 2,245 1 View

Is there a special procedure for cardiomyocyte isolation for diabetic and aging rat hearts?

We have been trying to isolate cardiomyocytes from type 2 diabetes model OLETF rats using conventional procedure for adult rat hearts but we always fail from obtaining viable cells. The rats are...

26 November 2014 6,827 8 View

Does inhibition of RyR2 by ryanodine always have to be through incubation of cells and not by perfusion?

I am not exactly familiar how ryanodine preparation is done, but I assumed I can perfuse it onto the cells during calcium transient measurement? But since its target is an intracellular component,...

28 October 2014 8,367 8 View

What could be the reason for getting an inconsistent calcium transient recording when using line scanning?

Hi! I frequently get an inconsistent recording during calcium transient recording using line scanning with a confocal microscope. I cannot get a baseline recording, but the cell does respond to...

27 September 2014 2,753 21 View

Has anyone experienced enlargement of the pulmonary artery after cannulation in the Langendorff system?

Has anyone else also experienced the increase in volume of the pulmonary artery after cannulation in a Langendorff apparatus? So, unlike other Langendorff systems, we only cannulate the aorta, and...

04 September 2014 5,088 14 View

Does anyone have experience with Kraft-Bruhe as storage solution for isolated cardiomyocytes?

So I am trying to isolate cardiomyocytes for calcium imaging purposes; however, I cannot get expected results with isoproterenol even at high concentration (1mM). It's definitely the cell...

28 August 2014 7,233 4 View