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Questions related from Julius Ryan Pronto
Is 2 weeks of high fat diet too short before STZ injection (low dose, 5 days)? My mice are weaned at 4 weeks and acclimatized to a normal diet 1 week before subjecting them to a high fat diet....
05 March 2018 6,407 7 View
My target proteins are RyR2 and SERCA2 and their respective regulators.
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I am not exactly familiar how ryanodine preparation is done, but I assumed I can perfuse it onto the cells during calcium transient measurement? But since its target is an intracellular component,...
28 October 2014 8,344 8 View
Hi! I frequently get an inconsistent recording during calcium transient recording using line scanning with a confocal microscope. I cannot get a baseline recording, but the cell does respond to...
27 September 2014 2,716 21 View
Has anyone else also experienced the increase in volume of the pulmonary artery after cannulation in a Langendorff apparatus? So, unlike other Langendorff systems, we only cannulate the aorta, and...
04 September 2014 5,060 14 View
So I am trying to isolate cardiomyocytes for calcium imaging purposes; however, I cannot get expected results with isoproterenol even at high concentration (1mM). It's definitely the cell...
28 August 2014 7,199 4 View