Julius Ryan Pronto

6 Questions 24 Answers 0 Followers

Questions related from Julius Ryan Pronto

What is the appropriate duration of high-fat diet prior to STZ injection in making type 2 diabetic ApoE knockout mice?

Is 2 weeks of high fat diet too short before STZ injection (low dose, 5 days)? My mice are weaned at 4 weeks and acclimatized to a normal diet 1 week before subjecting them to a high fat diet....

05 March 2018 6,408 7 View

What is the most reliable housekeeping gene for western blot analyses using sarcoplasmic reticulum vesicles?

My target proteins are RyR2 and SERCA2 and their respective regulators.

07 March 2016 2,170 1 View

Does inhibition of RyR2 by ryanodine always have to be through incubation of cells and not by perfusion?

I am not exactly familiar how ryanodine preparation is done, but I assumed I can perfuse it onto the cells during calcium transient measurement? But since its target is an intracellular component,...

28 October 2014 8,345 8 View

What could be the reason for getting an inconsistent calcium transient recording when using line scanning?

Hi! I frequently get an inconsistent recording during calcium transient recording using line scanning with a confocal microscope. I cannot get a baseline recording, but the cell does respond to...

27 September 2014 2,718 21 View

Has anyone experienced enlargement of the pulmonary artery after cannulation in the Langendorff system?

Has anyone else also experienced the increase in volume of the pulmonary artery after cannulation in a Langendorff apparatus? So, unlike other Langendorff systems, we only cannulate the aorta, and...

04 September 2014 5,063 14 View

Does anyone have experience with Kraft-Bruhe as storage solution for isolated cardiomyocytes?

So I am trying to isolate cardiomyocytes for calcium imaging purposes; however, I cannot get expected results with isoproterenol even at high concentration (1mM). It's definitely the cell...

28 August 2014 7,201 4 View