5 Questions 9 Answers 0 Followers
Questions related from Juliane Brandes
Hello, i have a question concerning the dissolution of Forskolin (abcam): We dissolved it in DMSO and PBS as stocksolution (100mM). (we used PBS, because of the adverse effects of DMSO on cell...
01 October 2021 4,412 3 View
Hello, I use the antibody P-PRAS40 in connection with isolated human monocytes. Among other things, I am investigating the status of P-PRAS40 using WES (capillary-based analysis of protein...
15 April 2021 7,727 2 View
Hello :) i have a question concerning LPS stimulation of monocytes: I investigate intracellular signalling (NFkB p65 for example) So I activate human PBMC monocytes with 50ng/ml LPS for 30 min....
26 March 2021 3,733 5 View
Hello :) does anyone of you have an established protcol for differentiation of murine peritoneal macrophages towards M1 and M2 macrophages? Or at least do you know a good paper? Thanks for any...
25 February 2021 5,932 6 View
Hello, do you know why monocytes (isolated from human PBMCs by CD14+beads) get less adherent in 6 well plates when they are incubated with LPS? Thanks Jule
23 November 2020 5,898 9 View
