Joshua Julian Glass

6 Questions 7 Answers 0 Followers

Questions related from Joshua Julian Glass

Is there a granulocyte population that expresses MHC-II in healthy individuals?

Hi All, I repeatedly find a minor subset of granulocytes in healthy human donors that stain for MHC-II (HLA-DR) quite above and distinct from the majority of granulocytes. Are any granulocyte...

05 May 2016 4,557 3 View

Where can I purchase monodisperse fluorescent polystyrene nanoparticles?

Hi All, I would like to purchase a range of fluorescent polystyrene nanoparticles that are quite monodisperse (narrow size distribution) and all within the nano size range. They are for immune...

01 January 2016 3,054 5 View

How do I perform confocal microscopy on non-adherent, fixed cells?

I'd like to perform confocal Z-stacks on a non-adherent/suspension cell line, following different nanoparticle treatments. How can I best 'restrain' these cells, so that they remain stationary...

05 May 2015 7,329 4 View

Best practice for analysing platelets by flow cytometry?

Hi All! I'm going to be analysing platelets by flow cytometry -- Simple surface staining. Does anyone have advice for separating platelets from blood? The need for platelet buffers? How to spin...

02 February 2015 2,534 10 View

How would you explain cell populations with negative values in flow cytometry plots?

Hi, Could someone please tell me what it means when there are 'very negative' populations in multi-colour (8+) flow cytometry? Example: I have attached a plot, with Cy5 on the x-axis. I...

01 January 2015 3,272 6 View

Would Protein A bind rat IgG with high enough affinity to not be competed off in human blood plasma?

Would Protein A bind a rat IgG antibody with high enough affinity to remain attached, not be competed off, when the Protein A-IgG is incubated in human blood plasma? Thanks in advance!

12 December 2014 6,796 6 View