Joshua Preston

3 Questions 2 Answers 0 Followers

Questions related from Joshua Preston

What type of insulin to use in in vitro vs. explant vs. in vivo models?

Hi all, Do you think it is appropriate to use Novolin R insulin (typically used for in vivo) for an in vitro or tissue explant model? I am trying to comb through methods when people have done...

05 December 2019 3,558 0 View

How to determine sex of de-identified RNA / cDNA from mouse fetal liver and placenta using qPCR?

I am trying to determine the sex of de-identified mouse placenta and fetal liver RNA / cDNA. Unfortunately, I do not have to tissue left to genotype the samples. I ran qPCR using a probe for two...

06 May 2019 2,371 0 View

Can you use 2^-Ct values to report qPCR results involving multiple tissue types where it is difficult to obtain a stable housekeeping gene?

I have dug through several threads on RG and found various opinions on this, but it appears that overall, doing qPCR comparison across tissues is messy business, because the more tissue types you...

16 November 2018 1,948 4 View