Josh Paster

3 Questions 5 Answers 0 Followers

Questions related from Josh Paster

Any advice on the trouble performing ELISPOT from cryopreserved samples?

PBMCs are preserved in 90 % FCS and 10 % DMSO, and kept in LN2 for extended periods of time. We routinely use simulators (hit with 3000 Rads) frozen under these conditions, with fresh responders...

11 November 2015 2,229 12 View

What is the best way to test for presence of anti-insulin antibody in diabetic cynomologous monkey?

I would like to determine the mechanism of diabetes development from one of our monkeys who had developed it as a result of treatment. We have hypothesized it being antibody mediated; does anyone...

05 May 2015 2,090 1 View

How long does Lithium-Heparinized Blood (NHP) stay "good" for?

We are receiving a shipment of blood for screening potential new monkeys in our facility, and due to weather complications they will sit for longer than we had hoped before they are processed. It...

01 January 2015 387 1 View