6 Questions 4 Answers 0 Followers
Questions related from Joseph Biwon
Hi, I am doing Percoll Gradient for C.novyi Spore purification, and my results are not consistent. I do 70%/55% as my gradient. I put about 3 mL of 70% Percoll, and above it, I layer about 6 mL...
25 October 2019 4,208 0 View
Hi, I understand plating diluted bacterial concentrations and counting the colonies give out the CFU value. But, how should I test the viability of bacterial samples with their initial...
23 October 2019 8,456 0 View
Hi, I am studying with Tramp-c1 cells, and have quick question on making cell culture medium for Tramp-C1. I have looked at the contents of complete growth medium at ATCC, but still have some...
01 May 2019 7,207 3 View
Hi, I thank you guys in advance for your helps always. I am in need of some clarifications in preparing cell injections from culture flasks. I have two t75 culture flasks, and I need to prepare 2...
16 April 2019 7,713 0 View
Hi, I am just curious as to why I should count the cell when doing cell passage. If I am subculturing just to maintain the cell line, I feel like there is no need to count the cell. For example,...
12 April 2019 2,090 1 View
Hi, I just have general question about passaging cells. I have two t75 flask of Tramp C1 culture, and when I am subculturing them, after all the necessary steps, I get 5 mL of media+trypsin from...
11 April 2019 4,814 4 View
