Joseph Biwon

4 Questions 3 Answers 0 Followers

Questions related from Joseph Biwon

Percoll Gradient for C. novyi Spore Purification?

Hi, I am doing Percoll Gradient for C.novyi Spore purification, and my results are not consistent. I do 70%/55% as my gradient. I put about 3 mL of 70% Percoll, and above it, I layer about 6 mL...

25 October 2019 4,171 0 View

How to Test Bacterial Viability?

Hi, I understand plating diluted bacterial concentrations and counting the colonies give out the CFU value. But, how should I test the viability of bacterial samples with their initial...

23 October 2019 8,428 0 View

Why is cell concentration important in cell passage?

Hi, I am just curious as to why I should count the cell when doing cell passage. If I am subculturing just to maintain the cell line, I feel like there is no need to count the cell. For example,...

12 April 2019 2,039 1 View

How to cell passage and calculate cell concentration?

Hi, I just have general question about passaging cells. I have two t75 flask of Tramp C1 culture, and when I am subculturing them, after all the necessary steps, I get 5 mL of media+trypsin from...

11 April 2019 4,788 4 View