Jooeun Lee

4 Questions 4 Answers 0 Followers

Questions related from Jooeun Lee

How do I search complete set of motifs of a particular protein (specifically, GIGANTEA from Arabidopsis thaliana)?

I am looking for the same from different bioinformatics websites like ExPASy, UniProtKB, etc but I'm unable to retrieve any successful data. I need an insight for this.

11 November 2019 3,670 2 View

Why did my LR White treatment not work efficiently?

This picture is from Arabidopsis thaliana leaf cells treated with LR White resin. As you can see cracks and shrinkage in the cells, which I think it was caused due to insufficient dehydration and...

11 November 2019 3,033 3 View

Can PBS be used for conducting plant based experiments. Any other suggestions to use as a buffer?

I'm planning to do immunofluorescence analysis for plant tissue(leaf). And I need to find an appropriate buffer to conduct the same. I used PBS for fixing, washing and diluting antibodies when I...

10 October 2019 5,239 3 View

Can LR white accelerator be used for Immuno-EM(Immuno-gold staining)?

Accelerator is used for polymerization of LR white resin which causes exothermic reaction. This affects the antigen structure. In order to prevent this from happening what precautions should I...

10 October 2019 4,539 1 View