Jon Huyghe

4 Questions 4 Answers 0 Followers

Questions related from Jon Huyghe

What do immunoglobulin variable region promoters look like?

I am trying to figure out how far the promoter/regulatory region of the immunoglobulin variable regions stretches upstream of the startcodon. I am aware that the conserved octamer sequence...

13 July 2017 4,531 2 View

IgM+ or IgG+ continuous B cell lines with sequenced immunoglobulin genes?

I am looking for an IgM+ or IgG+ continous B cell line, for which the immunoglobulin genes have been sequenced, preferentially the VDJ and VJ rearrangements. The cell line is preferentially from...

14 April 2017 3,928 4 View

What does the genome of hybridomas look like?

Is it correct that a hybridoma has the full intact genome of both the splenocyte and the myeloma to which it is fused? Or do genomic reorganizations take place post fusion? What about the genomic...

20 March 2017 6,622 2 View

VH and VL orientation for ScFv design in chimeric antigen receptor design?

Does the orientation of the VH and VL impact the binding properties of a ScFv when applied in a chimeric antigen receptor? Or are both configurations (VH-Linker-VL-CARbackbone vs....

13 October 2016 5,437 3 View