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Questions related from John Campbell McNamara
Recently, Christopher Monit, Richard A. Goldstein & Greg J. Towers published an article entitled 'ChromaClade: combined visualisation of phylogenetic and sequence data' in BMC Evolutionary...
18 November 2019 1,777 2 View
I need to add heparin to toad blood in the field for posterior biochemical analyses. Is there a standard anuran protocol for this? Is there an ideal concentration or volume of heparin per unit...
23 October 2018 6,875 0 View
Hi, the temperature sensor on my ancient 5417R has finally given up. Might anyone have a spare sensor, or a 5417R lying in disuse that could be scavenged from? Any help would be appreciated as the...
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We need to preserve crab hemolymph to evaluate acid-base characteristics at a later date. Will freezing and holding in liquid nitrogen allow this? Thanks.
22 December 2015 1,329 1 View
We want to eliminate potential contamination of plastic tubes and large pipette tips (5 mL). Will UV radiation disinfect the interior of the tubes and tips, passing through the plastic walls? Most...
01 July 2015 4,845 9 View
Has anyone been successful in amplifying the sodium-potassium-two chloride transporter in caridean shrimp gills? Could you provide the primer sequences please.
05 March 2015 2,452 2 View
In preparation for field studies, we have compared total RNA concentrations (by Qubit) in tissues directly extracted in Trizol with tissues extracted after holding for 48 hours in RNAlater buffer....
19 March 2014 7,317 19 View
We will shortly be engaged in fieldwork and we need to transport tissue samples (gill, muscle) back to the lab for RNA extraction and cDNA synthesis. I know RNALater is available for this but we...
19 December 2013 1,866 10 View
