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Questions related from Johannes Thomsen
Let's say you're developing an enzyme to catalyze a certain reaction. But the progress of this reaction is only measurable through e.g. fluorescently labelled substrate analogs, i.e. not actually...
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Obviously clean glassware is essential to the success and reproducibility of any experiment, but even more so for single molecule fluorescence microscopy which can be extremely sensitive to stray...
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Hi all. I'm doing some TIRF analysis of lipid membranes and layers, with and without dyes. I've long been pondering about some "strange" phenomena I've encountered. The internet isn't much help....
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I'm using the fluorophore Nile Red to image what happens to lipid samples over time in solution. Everywhere you look people talk about photobleaching, but in my case the sample gets brighter over...
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If I want to calculate the slope (velocity) of the absorbance curve at the linear region, how small/large should this region be for the most exact results? Should I go for the region where the...
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