Joe Davidson

6 Questions 2 Answers 0 Followers

Questions related from Joe Davidson

CRISPR Knock in Primary Human cell line?

Hi all, Hope you are well. I was wondering if someone could point me in the right direction of methods for protein knock in on a primary human cardiomyocyte cell line? I am not sure what would be...

19 May 2023 5,661 1 View

Crude mixture of fibroblasts, myocytes and smooth muscle cells following rat heart isolation?

Hello friends, I have been carrying out cardiomyocyte isolations for a few weeks now and I want to get a homologous mixture (or at least 90%+ purity) of just cardiomyocytes to carry out some...

16 February 2023 4,323 4 View

Analysing alphascreen cAMP assay data.

I am currently using Perkin and Elmer Alpha screen cAMP assay and I have been having issues analysing my data. I carried out my first experiment using HEK-293 cells and stimulating them with...

01 January 1970 5,314 1 View

IBMX stock solution

Hi all, I have a bit of a silly question. I was wondering the best way to make up a 100 mM IBMX stock? I have heard it is not very soluble and will require 70% ethanol and to be heated slightly....

01 January 1970 7,409 0 View

Running functional pharmacological assays after rat heart isolation.

Hi all, Hope you are well. I am in need of some help. I am currently exploring the effect of certain peptides on the production of cAMP in rat myocytes. I am using the Perkin and Elmer LANCE...

01 January 1970 9,791 0 View

Cardiomyocyte isolation problem

Hi, Hope you are all well. I have just started carrying out isolations and I am achieving relatively high yield of myocytes, but there are still a lot of dead cells present within my isolation...

01 January 1970 7,722 1 View