Jo Erickson

4 Questions 4 Answers 0 Followers

Questions related from Jo Erickson

Co-transfection optimisation for reporter plasmid and transcription factor plasmid?

I'm co-transfecting 1) a reporter plasmid with an inserted enhancer and the luciferase gene and 2) a plasmid with the Egr-1 transcription factor gene using jetPRIME reagent. I'm wondering what...

01 January 2020 3,331 5 View

Cell line recommendations for transfection of plasmid vector with human insert?

I have constructed a plasmid with a human DNA insert (5kb) that has the luciferase reporter gene. I will be looking to transfect this plasmid into mammalian cells and run a luciferase assay to...

01 January 2019 7,579 4 View

How to optimise PCR for linearising 4.2kb plasmid?

I'm trying to linearise a plasmid that I will use as my vector for NEBuilder HiFi DNA Assembly. I have designed primers for linearising the plasmid that I am now testing. There are shorter bands...

12 December 2018 4,591 4 View

Interpretation of Mouse Marble Burying

Using two different strains of KO mice I have completed running them through 'marble burying' as an indication for anxiety. The mice also went through an Elevated Zero Maze. I'm trying to figure...

01 January 1970 6,587 3 View