Jivan Shakya

5 Questions 7 Answers 0 Followers

Questions related from Jivan Shakya

How early can bacteria integrate spacer sequences into CRISPR loci?

I did some experiments infecting Salmonella Typhi with the lytic phage I isolated from the environment. It gives a nice clear plaques on agar overlay. However, in tube co-culture growth assay, the...

09 November 2019 5,013 5 View

How to deplete MDSCs in mice bearing 4T1 tumors?

I want to know the protocols to deplete MDSCs in mice bearing 4T1 tummors with particular emphasis on : 1. Which regimen of MDSC depletion (anti-Gr1 or Gemcitabine??) is better? 2. Dose and...

08 September 2016 8,497 4 View

Does IRES-eGFP have to be in frame with gene upstream in bicistronic construct?

Does the IRES-eGFP in the bicistronic construct be in-frame with the gene of interest upstream of it? I put my gene of interest upstream of EMCV-IRES-EGFP and realized that EGFP is in frame 2...

03 April 2016 3,913 4 View

How can I stably transfect 4T1 with gene of interest?

I want to be able to control the expression of protein of interest in 4T1 cell line and wanted to check if there could be a threshold of expression that determines the efficacy of vaccination...

01 December 2015 5,031 4 View

Why does serum has jelly-like consistency after separation in my experiment?

There's this problem I have been getting while separating the serum from blood collected from mice by terminal bleeding from heart. I collect 0.5-1.0ml blood, keep it in ice until centrifuguation...

02 July 2015 5,185 5 View