Jiaojiao Liu

5 Questions 6 Answers 0 Followers

Questions related from Jiaojiao Liu

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

23 February 2021 4,540 2 View

Is there some effective transfection reagents or methods for transfecting plasmids to ExpiCHO-S cells?

Dear all, We want to transfect pCDH plasmid or other plasmids to suspension ExpiCHO-S cells and express our target proteins, and we used the transfection reagents PEI. Unfortunately, there...

27 November 2020 8,528 3 View

Why are there so many platelets in PBMC of mice isolated by Ficoll, and What should I do to dislodge these paltelets?

Hi, We used Ficoll to isolate PBMC from mice whole blood cells, but there are many little nights,with no celler structures, in PBMC through a microscope. We guess these granules are paltelets, but...

30 September 2020 1,754 5 View

Why Neutralizing antibodies(NAbs) are important for viral clearance ? What's the difference betweent binding antibody and NAbs?

Hello, I am a postgraduate student and focused on vaccine research now, but I can't ultterly understanted these two terms and the difference between these two antibodies. Thanks a lot.

19 September 2020 3,160 3 View

How to analysis the data of RNA-Seq?

Hello, We used two sh-RNAs to konck-down a protein in MDA-MB-231 cells, and extract total RNA for RNA-Seq, and we got the results, but I don't know how to analysis these data. Dose there any tool...

08 January 2019 8,809 7 View