Jeffrey Vargas

6 Questions 1 Answers 0 Followers

Questions related from Jeffrey Vargas

Why my DNS (Dinitrosalicylic acid) don't react when I use a lyophilized bacterial supernatant to identify amylase reaction?

When I use the supernatant directly without lyophilized I have the change of color by the DNS (red-brown) but whe I use the lyophilized supernatant don´t have reaction, the control and the sample...

06 June 2017 3,078 1 View

Can anyone help me with a DNA extraction protocol for gram positive bacteria?

I need a DNA protocol extraction because a work with Arthrobacter genus bacteria and all the protocols I try result in a low concentration of DNA and high concentration of ARN.

03 March 2014 3,503 4 View

What is the best quantitative method to estimate the lactase activity in a lactose mineral medium?

I am testing with fehling reagent but I am not sure if this works well for this test.

03 March 2014 8,464 5 View

Anyone familiar with ARN in DNA digestion by restriction enzyme?

What happen if I have RNA fragments in my DNA digestion mix. Because I can´t eliminated the RNA in the DNA extraction. This interfered in the restriction enzyme reaction.

02 February 2014 1,912 5 View

How can I estimate the enzymatic kinetics?

I need to know if I can estimate the amylase enzymatic kinetics. I have data of absorbance and concentration and I have a standard curve of glucose (DNS) and starch (iodine solution).

02 February 2014 6,823 2 View

Can I extract genomic DNA from bacteria replaced the use Proteinase K by Sonication?

I need to know the risks of using sonication for my bacterial DNA. If I could use this, what time and frequency is necessary to sonicate in my bacterial samples?

01 January 2014 2,194 6 View