Jeff Schreifels

2 Questions 3 Answers 0 Followers

Questions related from Jeff Schreifels

What happens, expression wise, if I have 2 SV40 Poly A sequences back to back at the end of my gene?

Both of the SV40 Poly As are in the same direction and about 50 bp apart. When I cloned my donor plasmid I didn't realize there was a random sequence already there. Should I expect a significant...

01 August 2021 341 0 View

Why does nanodrop tell me I have plasmid but it does not show up on a gel?

I know this question has been asked before but I believe my issue is a bit different. I did a set of 6 minipreps from about 1.5mls of culture using the PureLink from Fisher. The nanodrop gave me...

19 November 2018 5,342 5 View