Jean Neil

3 Questions 3 Answers 0 Followers

Questions related from Jean Neil

How to set up the analysis for live/dead cells on facs?

I have isolated spleen cells and i want to check if i have live or dead cells. I have stained them with live dead aqua from invitrogen. I am using BD DIVA for facs. Which diagrams should i draw...

02 February 2018 4,266 0 View

Why do i have late amplification of my gene in qPCR even if i add more cDNA

I have done RNA isolation from a kidney cell line ( RIN 9.3), followed by reverse transcription using 1ug of RNA using iscript kit. But after qPCR, I found the positive control Ct value is 29...

07 July 2017 8,321 7 View

Why qPCR is showing a zigzag line instead of an amlification curve ?

qPCR was done to a gene called 36B4, 20ng of DNA and forward primer with concentration 300 nM and reverse primer of 500nM.qPCR machine used is lightcycler 480. Program used 95 c for 10 minutes,...

11 November 2016 8,843 9 View