Jason Saunders Williams

8 Questions 9 Answers 0 Followers

Questions related from Jason Saunders Williams

How do you analyse the expression of splice variants using TCGA data?

Hi everyone. I have been able to analyse the expression of genes using TCGA RNA seq data through the UCSC cancer browser. I can't find a way to analyse the expression of transcript variants of a...

04 May 2016 298 2 View

Why does LiCl ChIP wash buffer turns into gel at 4°C?

Hi all, I am using LiCl (500mM) wash buffer with 1% sodium deoxycholate and 1% SDS, 100mM Tris with Dynabeads. My protocol says to use it at 4°C, but when I tried this, the buffer turns into a...

15 March 2016 3,317 11 View

What is the best way to look for common functional polymorphisms in the regulatory region of a gene, i.e. using cancer genome databases?

I would like to look for functional polymorphisms upstream of the transcription start site of my gene of interest. Has anyone got any experience in doing this in populations of cancer patients?

18 February 2016 3,230 4 View

Does anyone have the cell line SNU-175?

Not available on ATCC

29 January 2016 1,667 1 View

Does anyone know of the best way to measure differences between samples (cancer cell lines) in the levels of ribosomal RNA (18S and 45S)?

I've tried qPCR with cDNA made from random hexamer and oligodT at the same time. Should I be using a ribosomal RNA primer to prime a total RNA extract? Also, what is the best way to quantify the...

10 December 2015 6,890 16 View

Chromatin shearing: Why am I always seeing long smears from which it is difficult to determine fragment sizes?

I have some problems visualising my sheared chromatin. I seem always to get a long smear from which it is difficult to determine fragement sizes. I am working with primary colorectal cancer cells...

28 October 2015 4,062 5 View

Does anyone know of an effective IHC protocol for use on Tissue Micro Arrays which reduces the loss of tissue?

I would like a protocol that maintains the fragile cores but reduces background and maintains effective antigen retrieval.

19 February 2015 7,178 8 View

Does anyone know where you can purchase a colon cancer associated fibroblast cell line?

I have a normal colon associated fibroblast cell line but I would also like a colon cancer associated fibroblast cell line to run along side in an alvetex invasion assay.

13 January 2015 1,877 3 View