Jasmin Acosta

3 Questions 1 Answers 0 Followers

Questions related from Jasmin Acosta

Can you thaw and refreeze human ascites?

Hi everyone, I'm troubleshooting using frozen ascites for different techniques i.e. cell culture and immunoblotting. However, when the ascites specimens were collected they were put at -80 C in...

08 November 2022 6,476 0 View

NK cell expansion protocol?

Hi all, It's my first time culturing NK cells, not only mine but also the first time it is being done in my lab so I am kind of on my own. Does anyone have a step-by-step protocol they could...

24 March 2022 5,981 1 View

Can you use Human Serum Albumin (HSA) instead of Human Serum (HS) in NK cell culture media?

I'm using the Miltenyi Biotec NK MACS medium however the protocol says to use 5% AB Serum, is it possible to substitute the HS for HSA? IL-2 and IL-15 will be added to the media to help with NK...

23 March 2022 6,180 3 View