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Questions related from Janine Dovey
I need to administer letrozole and/or dutasteride to mice via i.p. injection. I found papers dissolving dutasteride in saline + 0.3% gelatin, which I added at 1.25mg/ml. I found another paper...
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I am staining for PSD-95 in 30um brain slices and I wish to quantify the number of synaptic puncta. I've tried using the PunctaAnalyzer plugin for ImageJ and while it is useful for single cells,...
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I'm optimising the PSD-95 antibody 7E3 (Santa Cruz Biotechnology) to stain some 30um brain slices (resectioned from 300um). The current protocol I'm using has not been very successful in that I'm...
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