Ito Fernando

6 Questions 9 Answers 0 Followers

Questions related from Ito Fernando

What is the minimum number of treatments/levels to calculate LC50?

We conducted experiments to assess the toxicity of plant extracts against certain insect pests. We used three levels of concentration, i.e., 5, 7.5, and 10%, and a control treatment (0%). So,...

24 August 2023 7,485 3 View

How to quantify the actual amount of protein in a solution?

I use the Bradford method to quantify my protein with the Pierce BCA Kit assay. Particularly I use the microplate assay, 25 uL of a standard solution is mixed with 200 uL of a reagent, and after...

27 March 2019 2,478 8 View

What is the best solution/buffer to dissect small insect larvae and to isolate their midgut?

I want to extract the midgut of Callosobruchus maculatus larvae to isolate the protease. This will be my first time dissecting a very minute larvae (2nd/3rd instar). I read some publications about...

08 May 2018 1,141 2 View

Lophocateres pusillus, morphological differences of male and female ?

How to distinguish between male and female of Lophocateres pusillus? I wanna study about its biology e.g. the effect of different diets or temperature and RH to its growth, development, etc. Sadly...

31 March 2017 971 12 View

Lophocateres pusillus, what are the morphological differences between male and female ?

How to distinguish between male and female of Lophocateres pusillus? I want to study the insect biology e.g. the effect of different diets or temperature on its growth, development, etc. Sadly I...

31 March 2017 4,515 12 View

Does anyone have identification keys for Monochamus sp.?

I found this Monochamus in Indonesia, but i could not identify the species. Some Monochamus spp. are quarantine pests in Indonesia.

25 November 2015 8,515 4 View