Isabela Perez

5 Questions 4 Answers 0 Followers

Questions related from Isabela Perez

What are some improved protocol for RNA extraction from fish testis and semen?

I have tried to extract RNA from fish semen and testis using the RNEasy kit and also have the PureLink RNA kit. I have tried a few modifications I've found online, but the RNA comes either highly...

07 September 2023 7,644 2 View

Negative shRNA control reduces protein expression. Why?

I have transducted a cell line (TGBC2) with lentivirus with different shRNA sequences and controls. My controls were scramble and empty. The backbone is pLKO.1-puro. If it were just the shRNAs...

12 March 2020 3,654 2 View

Why does DMEM turn blue when you add chlorine?

I found some old DMEM tubes (10%SFB, 1%P/E) and I added a little bit of 10% chlorine, you know, of whatever household bleach brand. When I did so, after a few seconds, the medium turned...

06 November 2019 8,966 2 View

How can I improve my HEK293T transfection efficiency?

Hi all. I´m using calcium-phosphate transfection for 293T. I had never transfected cells with any method. I used two DNA concentrations, 5ug and 10ug per well (6-well plate). I expected lower than...

26 June 2019 9,694 5 View

Why can't chemically competent bacteria produce more competent bacteria?

I am using competent, commercially bought DH5a to obtain plasmids of interest. I understand that chemical treatment with CaCl2 or whatever other method used induces competency on treated bacteria...

04 April 2019 3,610 1 View