Irmak Bilgiseven

4 Questions 3 Answers 0 Followers

Questions related from Irmak Bilgiseven

Why would my comet assay of zebrafish blood have a cloudy appearance?

I did comet assay with goldfish in my master's degree but now I am experiencing such a problem. has anyone encountered such a problem before? the only difference from my other experiments is that...

10 May 2024 6,902 3 View

Why lots of nucleus appears red in fish erythrocytes when i am conduct micronucleus assay with acridine orange?

Hi everyone, i use acridine orange for micronucleus assau but lots of nucleus is red. Is anyone have this problem, can someone help me why all the cells red? Thanks so much for your time already.

25 October 2023 9,495 2 View

What is difference between Tris-Hcl buffer and phosphate buffer?

Protocols for GSH, SOD and CAT experiments treat tissue (fish liver tissue) with Tris-HCl buffer or phosphate buffer. Some articles say Tris-Hcl would be a better choice for ezimatic experiments....

13 February 2023 4,465 1 View

Can we use the same protocol for the microplate reader that is used for the spectrophotometer for antioxidant measurement ?

I want to look at the antioxidant changes (GSH, CAT, SOD measurements) in Goldfish (Carassius auratus). I found suitable protocols for spectrophotometer, can I also use them in microplate reader

15 December 2021 3,756 7 View