Indra Kusuma

7 Questions 34 Answers 0 Followers

Questions related from Indra Kusuma

What is you opinion in this picture i have of primary human keratinocytes?

I am trying to adapting primary human keratinocytes (p1) without collagen coating on the culture surface, so it is directly plating to the plastic These pic (1) after re-plating to plastic (P1)...

16 January 2017 9,409 9 View

Is this an alkaline phosphatase positive for stem cells?

We stain our fibroblast culture (designated for stem cell feeder cells) with AP, the results show a few red stained cells? is this a AP+ results or a false positive? is this means that there is a...

29 December 2015 6,517 6 View

Can anyone help me with my primary keratinocyte culture that always dies out after 7 days (detailed protocol listed below)?

fresh foreskin, short decontamination procedure using povidone iodine and EtOh, overnight 4 C incubation in dispase II 1 ug/ml diluted in PBS w/ Ca, Mg for dermal-epidemal separation, epidermal...

21 January 2015 1,788 4 View

Is anyone currently working with trehalose loading to mammalian cells?

We try to load trehalose inside cytoplasm, want kind of technique currently available and discussed, how to control the process and evaluate the results

02 September 2014 9,699 3 View

reconstitution tutorial

Hi there..this is my first time to handle reagents..we just bought Hydrocortisone and EGF from Sigma for our cell culture project problem is i can't figured out how to reconstitute them, the...

04 September 2010 9,865 8 View

setting up new lab

hi evryone.. i am setting up a new lab for cell culture, does anyone know what is the composition of Oxygen, CO2 and N2 inside the incubator

26 July 2010 6,122 6 View

Keratinocytes culture problem

I have succesfully isolated and cultured human basal keratinocytes from foreskin for about a year now. Lately I encountered a puzzling problem - for the last isolation I managed to isolate the...

01 January 1970 5,896 17 View