Ina Laura Pieper

4 Questions 14 Answers 0 Followers

Questions related from Ina Laura Pieper

What do you think of this centrifugation protocol for preparing plasma samples for microparticle analysis by flow cytometry from peripheral blood?

Peripheral blood tubes are immediately inverted 10 times and centrifuged at 4,200 x g, 7 min, with brake. The supernatant plasma is transferred to a new tube and centrifuged at 13,000 x g, 5 min...

15 January 2015 9,748 4 View

What's the best activation marker antibodies to use for pig platelets?

I'm planning to investigate the activation status of porcine (pig) platelets as a response to various stimuli. What antibodies would you recommend for flow cytometry?

13 February 2014 9,575 5 View

Can someone recommend a multicolour immune cell activation flow cytometry panel?

I'm looking to monitor immune cell activation (neutrophils, monocytes, T/B/NK-cells) using multicolour flow cytometry and was wondering if anyone can recommend already established panels?

08 February 2014 4,120 10 View

What's the best plate-based assay for von Willebrand factor (vWF) activity?

I've used the multimer immunoblot analysis and the flow cytometry ristocetin-based platelet aggregation assay, but these are both time- and labour-intensive methods. Can you recommend a...

04 February 2014 7,105 2 View