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Questions related from Iason Karyofyllis
So another PCR problem. I have to amplify a 3 kbp construct with high GC content. Generally all the constrcut isaround 63%. My construct consists of the pcagpromoter with the cmv enchancer and a...
11 November 2018 467 9 View
I have a plasmid with the correct donor sequence. I want to use Crispr to insert it in frame . So in order to do that and lessen my cloning steps, I have to use Gibson Assembly for the insert...
06 June 2018 2,880 1 View
well i want to amplify the a pcr product of 2kb. i am using Phusion (master mix) from Thermofischer . So I have this plasmid (addgene:28304) http://www.addgene.org/28304/ and my primers are the...
05 May 2018 10,050 3 View
