Hong Huang

5 Questions 4 Answers 0 Followers

Questions related from Hong Huang

How to explain the elevation of targeted gene expression on protein level but not mRNA level?

Recently we overexpressed a targeted gene on the lentivirus vector. However, through repeated analysing, the elevation was validated on protein level by WB while not on mRNA level by qRT-PCR....

21 September 2021 1,104 6 View

Will blood loss by trauma influence wound repair?

I wonder if huge amount of blood loss (20-40%) would affect wound healing? Wound healing needs stem cells to migrate to the injured area, whether hemorrhage is a barrier to the migration and...

11 November 2014 7,313 7 View

How can I lower the modality of diabetic rat with STZ (65mg/kg)?

hello, I recently was trying to make type II diabetic rats model by i.p injection STZ (65mg/kg). But the rats was dying in 5 days even I tried to give little insulin(4 IU) everyday since day 3 to...

10 August 2014 2,230 6 View

Can anyone help me with some suitable markers to identify regenerative skeletal muscle cells?

I started some work on skeletal muscle repair and regeneration recently. Due to lack of experience, I'm confused at choosing the cell surface marker to identify regenerated muscle cells in rats...

01 August 2014 7,047 7 View

How to explan the relationship of increased m6A level by Mettl3 and higher expressed gene（hypomethylated）

As a significant increase of m6A level in response to hypertrophic stimulation, and METTL3-mediated methylation of mRNA is enhanced in response to hypertrophic stimuli and is necessary for a...

01 January 1970 6,638 0 View