Hisataka Ogawa

3 Questions 8 Answers 0 Followers

Questions related from Hisataka Ogawa

How long are cytokines stable in the fridge ?

Hello I am just wondering how long cytokines can be undegraded in the fridge. In culturing T cells, I usually add fresh cytokines into culture media. Some researchers say it"s OK to make culture...

29 March 2017 7,268 4 View

Do you have any knowledge about electroporation into PBMC ?

Hello I am trying to electroporate DNA into freash PBMC using ECM830 Electro Square Porator (Harvard Apparatus BTX). I am running optimization with various options like voltage, pulse length and...

15 March 2017 172 8 View

Optimal 5-FU dose in tumor bearing NSG mice ?

I am working on cancer immunotherapy using orthotopically implanted NSG mice model. As you know, NSG mice are so sensitive to stress including anti-cancer drug (5-FU) so I want to strike a happpy...

01 January 1970 7,884 2 View